Question

Each of the given statements describes a type of column chromatography. Match the statements to the type of chromatography they describe. If a statement can describe all of the types, place that statement in the All category. (Note that size‑exclusion chromatography may also be called gel filtration or molecular‑exclusion chromatography.)

Categories: Size-exclusion Chromatography, Affinity Chromatography, Ion-exchange Chromatogrpahy, and All

Statements:
(a) Separate molecules by size.
(b) Separate molecules by charge.
(c) The stationary phase has a covalently bound group to which a protein in the mobile phase can bind.
(d) Uses a mobile phase and a stationary phase to separate proteins.
(e) The stationary phase contains cross-linked polymers with different pore sizes.
(f) Can separate molecules based on protein-lignand binding.
(g) The stationary phase may contain negatively or positively charged groups.

Answer 1

Answer:

The proper matching is given below.

Explanation:

a Separate molecules by size    size exclusion chromatography

b Separate molecules by charge Ion exchange chromatography

c The stationary phase has a covalently bound group to which a protein in the mobile phase can bind.                  Affinity chromatography

d uses mobile phase and stationary phase to separate protein Size exclusion chromatography

e The stationary phase contain  cross linked polymers with different pore size

                                                                  Size exclusion chromatography

f can separate molecules based on protein ligand binding   Affinity chromatography

g The stationary phase may contain negatively or positively charged groups

  ion exchange chromatography