Which of the following best describes the fate of the cloned DNA used in a transformation experiment?
a. The DNA is integrated into a chromosome and maintained indefinitely.
b. The DNA remains on a plasmid and is maintained as an episome (non-chromosomal DNA) indefinitely.
c. The DNA remains on a plasmid and is maintained as an episome (non-chromosomal DNA) for a day or two and is then lost.
d. The DNA is integrated into a chromosome but is degraded and lost after a day or two.

Question

allisonchorath5872 allisonchorath5872

01-11-2019
Biology

contestada

Which of the following best describes the fate of the cloned DNA used in a transformation experiment?
a. The DNA is integrated into a chromosome and maintained indefinitely.
b. The DNA remains on a plasmid and is maintained as an episome (non-chromosomal DNA) indefinitely.
c. The DNA remains on a plasmid and is maintained as an episome (non-chromosomal DNA) for a day or two and is then lost.
d. The DNA is integrated into a chromosome but is degraded and lost after a day or two.

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pandeyarindam296 pandeyarindam296 · Answer 1 · 2019-11-05T14:13:41+01:00

Answer:

The correct answer is a DNA is integrated into a chromosome and maintained indefinitely.

Explanation:

During DNA Cloning the target DNA is cleaved by restriction endonuclease result in the generation of target DNA fragments.

A suitable vector is chosen and the DNA of that vector is cut with same restriction enzyme which lead to generate a gap in the vector DNA.

A target DNA fragment is then integrated with the gap region of the vector and is maintained indefinitely .