A common column material used in size-exclusion chromatography is dextran, a polysaccharide of glucose. Which type of interaction most likely occurs between proteins and the dextran column material?
A hydrogen bond is a form of the electrostatic force of attraction which takes place between the hydrogen atom and a more electronegative atom such as nitrogen, fluorine, and oxygen.
Hydrogen bond falls in the category of weak forces.
In size-exclusion chromatography, the type of interaction that takes place between the proteins and dextran is hydrogen bonding.
The hydrogen bonding is possible because dextran, being a polysaccharide of glucose has several hydroxyl groups and thus, they get involved in the hydrogen bonding.
