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The main difference between eukaryotic and prokaryotic cells is that eukaryotic cells have a nucleus. The nucleus is where cells store their DNA, which is the genetic material. The nucleus is surrounded by a membrane. Prokaryotic cells do not have a nucleus. Instead, their DNA floats around inside the cell.

The correct way of distinguishing an unknown microscopic organism to be classified as a prokaryote or eukaryote is to observe the unknown sample under a microscope.

Matching the observed cellular components with the characteristic structural components of eukaryotic or prokaryotic cells as eukaryotic and prokaryotic cells have some characteristic differences.

Eukaryotic cells:

  • true nucleus present around nucleoplasm
  • double membrane-bound cellular organelles present
  • vacuole present
  • cell wall may or may not be present
  • cytoplasmic membrane present
  • microtubules present
  • lysosomes present

Prokaryotic cells:

  • true nucleus absent
  • double membrane-bound cellular organelles absent
  • vacuole absent
  • cell wall present
  • cytoplasmic membrane absent
  • microtubules absent
  • lysosomes absent
  • pili or flagella present

There are other methods also present such as dichotomous key, comparing the unknown sample with the known sample of both types.

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