We have two subsequent enzymatic reactions in the pathway leading from primary substrate to a final product through the intermediate metabolite. The Km of the enzyme in the first reaction is lower than the Km in the second reaction. What is reasonable to expect?
1) Smooth flux - equal rates in both reactions
2) First reaction will proceed slow, while the second reaction will proceed fast
3) Accumulation of the intermediate
4) Change in equiLiBrium between primary substrate and final product
