You are planning to do a ligation between a 1 kb insert and a 2 kb plasmid. Your goal is to set up a 1:1 molar ratio between the insert and the vector. Which of the following would work?
1) 1 µg of insert and 2 µg of vector
2) 1 µg of insert and 1 µg of vector
3) 2 µg of insert and 1 µg of vector
4) 2 µg of insert and 2 µg of vector
