Treatment of hemoglobin with low concentrations of urea (a chaotropic agent that at high concentrations will completely denature proteins) results in the disruption of the α and β-subunit interfaces. This disruption will cause all four subunits to dissociate resulting in the lost of the normal quaternary structure hemoglobin exhibits, BUT the subunits will retain their tertiary structure and will still function in oxygen binding. Given this information complete the following two problems: A) State under which conditions hemoglobin will have the greatest affinity for oxygen. For this you can simply state "Urea" or "Normal". B) Now state WHY you think hemoglobin has a lower or higher affinity for oxygen when placed in a solution with low concentrations of urea
