The enzyme lactate dehydrogenase catalyzes the reaction; Pyruvate + NADH + H⁺ → lactate + NAD⁺. NADH absorbs light at 340 nm in the near-ultraviolet region of the electromagnetic spectrum, but NAD⁺ does not. Suggest an experimental method for following the rate of this reaction, assuming that you have available a spectrophotometer capable of measuring light at this wavelength.
