Precise measurement with a pipette is technique dependent in most cases. The following is a list of best practices in pipetting. Which one of the following could be detrimental to accurate pipetting?
1) Invert or lay the pipette on its side while there is liquid in the tip
2) Pre-wet the pipette tip 3 times prior to delivering the specified sample volume.
3) Develop a steady aspiration and dispensing cadence that emphasizes speed and skill.
4) Allow all sample fluids and equipment to reach room temperature prior to pipetting.
