Calculate the transformation efficiency of the following experiment using the information and results listed below: DNA plasmid concentration 0.08ug/ul 250ul CaC12 transformaiton solution 10ul pGLO plasmid solution 250ul LB Broth 100ul cells spread on agar 227 colonies of transformants Show your calculations, and answer the following: 1. What number of colonies were on the LB/amp/ara plate? 2. How much DNA was spread on the plate in ug? 3. What is the transformation efficiency? If a particular experiment were known to have 3x10 bacteria/ug of DNA, how many transformant colonies would be expected to grow on the LB/amp/ara plate? You can assume that the concentration of DNA and fraction of cells spread on the LB agar are the same as that of the pGLO laboratory.
