Fill in each blank with the best word or phrase selected from the list below. Not all words or phrases will be used; each word or phrase can be used only once. charged pause site lytic mismatch promoter RBS clear origin - strand tRNA phosphorylation + strand lysogenic ppGpp uncharged sigma factor Rho factor methylation pause site cloudy Before transcription can begin, RNA polymerase must find the location and direction of a gene on the chromosome based on its sequence. RNA polymerase ends transcription at a located at the end of a gene. It is common for amino acid biosynthetic genes to be transcriptionally activated in the presence of tRNAs as a result of the Stringent response, which requires to bind RNA polymerase. An sRNA can inhibit mRNA translation by the ribosome through complementary base-pairing to the sequence in its target mRNA. During DNA replication, the sequence of the daughter strand being synthesized is determined by complementary base-pairing with the parent template strand, however DNA polymerase can incorporate a base-pair that can result in a point mutation if it is not repaired. a Bacteria can repair these mistakes and distinguish the original parent strand of DNA from the new daughter strand of DNA through of certain nucleotides. In order to replicate their genomes, all SSRNA viruses must package an RNA-dependent RNA polymerase within their capsid.