Arrange the following in order of increasing Rf on thin-layer chromatography, acetic acid, acetaldehyde, 2-octanone, decane, and 1-butanol. 2. What problem will ensue if the level of the developing liquid is higher than the applied spot in a TLC analysis? 3. In what order (from top to bottom) would you expect to find naphthalene, butyric acid, and phenyl acetate on a silica gel TLC plate developed with dichloromethane? 4. Why might it be very difficult to visualize the separation of cis- and trans-2-buttne by thin-layer chromatography? 5. Please calculate Rf value of three samples. 00 Substance B 7.8 cm 4.5 cm 0 Substance A 1.3 cm 1 2 3
