Ligase catalyzes a reaction between the 5'-phosphate and the 3'-hydroxyl at the ends of DNA molecules. The enzyme calf intestinal phosphatase catalyzes the removal of the 5'-phosphate from DNA molecules.
What would be the consequence of treating the vector, before ligation, with calf intestinal phosphatase?
A) It would prevent the ends of the plasmids from being ligated.
B) It would prevent the plasmid's DNA from reversing polarity during ligation.
C) It would reverse the polarity of plasmids' DNA
D) It would ligate the ends of the plasmids.
A) It would prevent the ends of the plasmids from being ligated.
