5. You are asked to determine the number of LIVE bacteria in a stream at noon each day for the month of August. The July study showed about 1 LIVE CELL (colony-forming unit) PER 20 ml OF STREAM WATER. There is no reason to believe that the August numbers will be much different. Which of the following methods will allow you to accomplish this task?
a Micropore filtration and plating the filter on a solid medium.
b. Serial dilution and plating.
c. Placing 100 ml in a sterile broth, then plating the incubated culture.
d. Determining the dry weight of a 100 ml sample.
e. Using a microscope and a counting chamber
6. Let us assume that you are working with a bacterial culture where cells do not cling together after division (they remain uniformly suspended in the broth). Or the following. which one is an APPROPRIATE (correct) REASON for you to use the ELECTRONIC PARTICLE COUNTER?
a. samples are processed with great speed
b. equipment is very inexpensive
c. will allow you to differentiate between live and dead cells
d. will give accurate determination of viable numbers
e. will let you measure lag, exponential, stationary, and death phases and death phases
7. A culture in a closed vessel to which no additional medium is added and from which no waste products are removed is called a ______ culture.
a continuous
b. hatch
c. fed-batch
d. semicontinuous
8. Which of the following is the type of reproduction found in most unicellular procaryotic microorganisms?
a. meiosis
b. mitosis
c. fragmentation
d. budding
e. binary fission
